The Effect of Voltage-Sensitive Dye di-4-ANEPPS on Heart Rate Variability in Langendorff-Perfused Isolated Rabbit Heart

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JANOUŠEK Oto RONZHINA Marina HEJČ Jakub OLEJNÍČKOVÁ Veronika STRAČINA Tibor FIALOVÁ Kateřina NOVÁKOVÁ Marie PROVAZNÍ Ivo KOLÁŘOVÁ Jana

Rok publikování 2015
Druh Článek ve sborníku
Konference Computing in Cardiology 2015
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Obor Fyziologie
Klíčová slova di-4-ANEPPS;Voltage-Sensitive Dye
Popis Optical mapping of heart electrical activity in Langendorff-perfused isolated hearts is based on voltagesensitive dyes, of which the most commonly used one is di-4-ANEPPS. Prolongation of action potential duration by di-4-ANEPPS was reported in isolated cells; however, this phenomenon was not observed in the whole heart model. In order to search for other adverse phenomena of di- 4-ANEPPS use, its effect on heart rate variability (HRV) was investigated during staining and washout periods in five New Zealand White rabbit isolated hearts. Time domain, frequency domain and non-linear HRV parameters (21 in total) revealed that there are no significant changes between control, staining and washout periods. Voltage-sensitive dye di-4-ANEPPS does not affect HRV when applied into the isolated heart coronary system. It can be concluded that di-4-ANEPPS may be safely used in studies combining optical mapping and HRV analysis
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