Electrochemical sensing of etoposide using carbon quantum dot modified glassy carbon electrode

Autoři	NGUYEN Hoai Viet RICHTERA Lukas MOULICK Amitava XHAXHIU Kledi KUDR Jiri CERNEI Natalia POLANSKÁ Hana HEGER Zbynek MASAŘÍK Michal KOPEL Pavel STIBOROVA Marie ECKSCHLAGER Tomas ADAM Vojtech KIZEK Rene
Rok publikování	2016
Druh	Článek v odborném periodiku
Časopis / Zdroj	Analyst
Fakulta / Pracoviště MU	Lékařská fakulta
Citace
Doi	http://dx.doi.org/10.1039/c5an02476e
Obor	Biochemie
Klíčová slova	PERFORMANCE LIQUID-CHROMATOGRAPHY; DNA TOPOISOMERASE-II; HUMAN PLASMA; CATECHOL METABOLITE; EMERGENT NANOLIGHTS; ANTITUMOR AGENTS; VP 16-213; NANOPARTICLES; DRUGS; CELLS
Popis	In this study, enhancement of the electrochemical signals of etoposide (ETO) measured by differential pulse voltammetry (DPV) by modifying a glassy carbon electrode (GCE) with carbon quantum dots (CQDs) is demonstrated. In comparison with a bare GCE, the modified GCE exhibited a higher sensitivity towards electrochemical detection of ETO. The lowest limit of detection was observed to be 5 nM ETO. Furthermore, scanning electron microscopy (SEM), fluorescence microscopy (FM), and electrochemical impedance spectroscopy (EIS) were employed for the further study of the working electrode surface after the modification with CQDs. Finally, the GCE modified with CQDs under optimized conditions was used to analyse real samples of ETO in the prostate cancer cell line PC3. After different incubation times (1, 3, 6, 9, 12, 18 and 24 h), these samples were then prepared prior to electrochemical detection by the GCE modified with CQDs. High performance liquid chromatography with an electrochemical detection method was employed to verify the results from the GCE modified with CQDs.