RECQ5 Helicase Cooperates with MUS81 Endonuclease in Processing Stalled Replication Forks at Common Fragile Sites during Mitosis

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DI MARCO Stefano HAŠANOVÁ Zdenka KANAGARAJ Radhakrishnan CHAPPIDI Nagaraja ALTMANNOVÁ Veronika MENON Shruti SEDLÁČKOVÁ Hana LANGHOFF Jana SURENDRANATH Kalpana HÜHN Daniela BHOWMICK Rahul MARINI PALOMEQUE María Victoria FERRARI Stefano HICKSON Ian D. KREJČÍ Lumír JANSCAK Pavel

Rok publikování 2017
Druh Článek v odborném periodiku
Časopis / Zdroj Molecular Cell
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.1016/j.molcel.2017.05.006
Obor Biochemie
Klíčová slova common fragile sites; genomic instability; mitotic DNA synthesis; MUS81; RAD51 filament; RECQ5; replication stress
Popis The MUS81-EME1 endonuclease cleaves late replication intermediates at common fragile sites (CFSs) during early mitosis to trigger DNA-repair synthesis that ensures faithful chromosome segregation. Here, we show that these DNA transactions are promoted by RECQ5 DNA helicase in a manner dependent on its Ser727 phosphorylation by CDK1. Upon replication stress, RECQ5 associates with CFSs in early mitosis through its physical interaction with MUS81 and promotes MUS81-dependent mitotic DNA synthesis. RECQ5 depletion or mutational inactivation of its ATP-binding site, RAD51-interacting domain, or phosphorylation site causes excessive binding of RAD51 to CFS loci and impairs CFS expression. This leads to defective chromosome segregation and accumulation of CFS-associated DNA damage in G1 cells. Biochemically, RECQ5 alleviates the inhibitory effect of RAD51 on 30-flap DNA cleavage by MUS81-EME1 through its RAD51 filament disruption activity. These data suggest that RECQ5 removes RAD51 filaments stabilizing stalled replication forks at CFSs and hence facilitates CFS cleavage by MUS81-EME1.
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