Dual-color quantum dots-based simultaneous detection of HPV-HIV co-infection


JIMENEZ Ana Maria Jimenez MOULICK Amitava RICHTERA Lukas KREJCOVA Ludmila KALINA Lukas DATTA Rahul SVOBODOVÁ Markéta HYNEK David MASAŘÍK Michal HEGER Zbynek ADAM Vojtech

Rok publikování 2018
Druh Článek v odborném periodiku
Časopis / Zdroj Sensors and Actuators B: Chemical
Fakulta / Pracoviště MU

Lékařská fakulta

Doi http://dx.doi.org/10.1016/j.snb.2017.11.074
Klíčová slova Electrochemistry; Human immunodeficiency virus; Human papillomavirus; Nanotechnology; Magnetic isolation; Quantum dots
Popis Human papillomavirus (HPV) and human immunodeficiency virus (HIV) are closely related infections; both diseases are mutually reinforcing due to the co-infection effects, which increase their clinical and epidemiological implications, raising urgent public health concerns. In the present study, a nano-system was developed for simultaneous detection of HPV and HIV targeting E6-HPV16 and Gag-HIV genes respectively. The target sequences from the samples of the patients suffering from head and neck cancer were isolated by magnetic glass particles (MGPs) and subsequently were hybridized with a mixture of the complementary probes attached to cadmium telluride quantum dots (CdTe QDs). Finally, the specific and successful binding was determined by an easy fluorescence analyses and was corroborated by PCR and electrochemistry. Red and green CdTe QDs were used for the dual-color detection of HPV and HIV respectively. Our nano-system can be applied as a proficient tool for the identification of HPV, HIV or both diseases simultaneously, getting a successful and high sensitivity system of detection. (C) 2017 Elsevier B.V. All rights reserved.

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