The Effect of Synthesis Procedure on Hydrogen Peroxidase-Like Catalytic Activity of Iron Oxide Magnetic Particles

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Publikace nespadá pod Lékařskou fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.
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MUKHERJEE A. ASHRAFI A.M. SVEC P. RICHTERA L. PŘIBYL Jan BRTNICKY M. KYNICKY J. ADAM V.

Rok publikování 2020
Druh Článek v odborném periodiku
Časopis / Zdroj Applied Sciences-Basel
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www https://www.mdpi.com/2076-3417/10/19/6756
Doi http://dx.doi.org/10.3390/app10196756
Klíčová slova magnetic nanoparticle; nanozyme; chronoamperometry; biosensor; peroxidase-like activity
Popis A comparative study was carried out using magnetic nanoparticles (MNPs) for the fabrication of non-enzymatic sensors for the continuous and rapid detection and monitoring of H2O2. Various MNPs, differing in terms of their synthesis procedure and modification, were synthesized and characterized by different techniques. The electrochemical catalytic activity of the synthesized MNPs toward the reduction in H2O2 was investigated by cyclic voltammetry. The naked MNPs showed the highest catalytic activity among all the synthesized MNPs. The biosensor based on the naked MNPs was then applied in the determination of H2O2 using chronoamperometry. The parameters such as the applied cathodic potential and the amount of MNPs on the developed biosensor were optimized. Moreover, the analytical figures of merit, including reproducibility (RSD = 6.14%), sensitivity (m = 0.0676 mu A mu M-1), limit of detection (LOD) = 27.02 mu mol L-1, and limit of quantification (LOQ) = 89.26 mu mol L-1 of the developed biosensor indicate satisfactory analysis. Finally, MNPs were successfully utilized for the determination of H2O2 in milk.
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