Analysis of Treponema pallidum Strains From China Using Improved Methods for Whole-Genome Sequencing From Primary Syphilis Chancres

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CHEN W. T. ŠMAJS David HU Y. F. KE W. J. POSPÍŠILOVÁ Petra HAWLEY K. L. CAIMANO M. J. RADOLF J. D. SENA A. TUCKER J. D. YANG B. JULIANO J. J. ZHENG H. P. PARR J. B.

Rok publikování 2021
Druh Článek v odborném periodiku
Časopis / Zdroj The Journal of Infectious Diseases
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www https://academic.oup.com/jid/article-abstract/223/5/848/5876405?redirectedFrom=fulltext
Doi http://dx.doi.org/10.1093/infdis/jiaa449
Klíčová slova chancre; China; syphilis; Treponema pallidum; whole-genome sequencing
Popis Background. Whole-genome sequencing (WGS) of lreponema pallidum subspecies pallidum (TPA) has been constrained by the lack of in vitro cultivation methods for isolating spirochetes from patient samples. Methods. We built upon recently developed enrichment methods to sequence TPA directly from primary syphilis chancre swabs collected in Guangzhou, China. Results. By combining parallel, pooled whole-genome amplification with hybrid selection, we generated high-quality genomes from 4 of 8 chancre-swab samples and 2 of 2 rabbit-passaged isolates, all subjected to challenging storage conditions. Conclusions. This approach enabled the first WGS of Chinese samples without rabbit passage and provided insights into TPA genetic diversity in China.
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