Sensitivity to Cisplatin in Head and Neck Cancer Cells Is Significantly Affected by Patient-Derived Cancer-Associated Fibroblasts

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PELTANOVÁ Barbora LIŠKOVÁ Markéta GUMULEC Jaromír RAUDENSKÁ Martina HOLCOVÁ POLANSKÁ Hana VACULOVIČ Tomáš KALFERT David GREGA Marek PLZAK Jan BETKA Jan MASAŘÍK Michal

Rok publikování 2021
Druh Článek v odborném periodiku
Časopis / Zdroj International Journal of Molecular Sciences
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www https://www.mdpi.com/1422-0067/22/4/1912
Doi http://dx.doi.org/10.3390/ijms22041912
Klíčová slova head and neck cancer; cancer-associated fibroblasts; cisplatin; treatment resistance; cancer recurrence; patient-derived cell cultures; coculture
Popis Cancer-associated fibroblasts (CAFs) are one of the most abundant and critical components of the tumor stroma. CAFs can impact many important steps of cancerogenesis and may also influence treatment resistance. Some of these effects need the direct contact of CAFs and cancer cells, while some involve paracrine signals. In this study, we investigated the ability of head and neck squamous cell carcinomas (HNSCC) patient-derived CAFs to promote or inhibit the colony-forming ability of HNSCC cells. The effect of cisplatin on this promoting or inhibiting influence was also studied. The subsequent analysis focused on changes in the expression of genes associated with cancer progression. We found that cisplatin response in model HNSCC cancer cells was modified by coculture with CAFs, was CAF-specific, and different patient-derived CAFs had a different "sensitizing ratio". Increased expression of VEGFA, PGE2S, COX2, EGFR, and NANOG in cancer cells was characteristic for the increase of resistance. On the other hand, CCL2 expression was associated with sensitizing effect. Significantly higher amounts of cisplatin were found in CAFs derived from patients who subsequently experienced a recurrence. In conclusion, our results showed that CAFs could promote and/or inhibit colony-forming capability and cisplatin resistance in HNSCC cells via paracrine effects and subsequent changes in gene expression of cancer-associated genes in cancer cells.
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