Septin-microtubule association via a motif unique to isoform 1 of septin 9 tunes stress fibers

Autoři	KUZMIĆ M. CASTRO LINARES G. LEISCHNER FIALOVÁ Jindřiška IV F. SALAÜN D. LLEWELLYN A. GOMES M. BELHABIB M. LIU Y. ASANO K. RODRIGUES M. ISNARDON D. TACHIBANA T. KOENDERINK G. H. BADACHE A. MAVRAKIS M. VERDIER-PINARD P.
Rok publikování	2022
Druh	Článek v odborném periodiku
Časopis / Zdroj	Journal of Cell Science
Fakulta / Pracoviště MU	Lékařská fakulta
Citace
www	https://journals.biologists.com/jcs/article-abstract/135/1/jcs258850/273936/Septin-microtubule-association-via-a-motif-unique
Doi	http://dx.doi.org/10.1242/jcs.258850
Klíčová slova	Actin; Cytoskeleton; Microtubule; Septin; SEPT9
Popis	Septins, a family of GTP-binding proteins that assemble into higher order structures, interface with the membrane, actin filaments and microtubules, and are thus important regulators of cytoarchitecture. Septin 9 (SEPT9), which is frequently overexpressed in tumors and mutated in hereditary neuralgic amyotrophy (HNA), mediates the binding of septins to microtubules, but the molecular determinants of this interaction remained uncertain. We demonstrate that a short microtubule-associated protein (MAP)-like motif unique to SEPT9 isoform 1 (SEPT9_i1) drives septin octamer-microtubule interaction in cells and in vitro reconstitutions. Septin-microtubule association requires polymerizable septin octamers harboring SEPT9_i1. Although outside of the MAP-like motif, HNA mutations abrogate this association, identifying a putative regulatory domain. Removal of this domain from SEPT9_i1 sequesters septins on microtubules, promotes microtubule stability and alters actomyosin fiber distribution and tension. Thus, we identify key molecular determinants and potential regulatory roles of septin-microtubule interaction, paving the way to deciphering the mechanisms underlying septin-associated pathologies.