Serum and cerebrospinal fluid phosphorylated neurofilament heavy subunit as a marker of neuroaxonal damage in tick-borne encephalitis.

Varování

Publikace nespadá pod Lékařskou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.

Autoři	FOŘTOVÁ Andrea HÖNIG Václav PALUS Martin SALÁT Jiří PÝCHOVÁ Martina KRBKOVÁ Lenka VYHLÍDALOVÁ Tereza KŘÍHA Michal F. CHRDLE Aleš RŮŽEK Daniel
Rok publikování	2022
Druh	Článek v odborném periodiku
Časopis / Zdroj	Journal of General Virology
Fakulta / Pracoviště MU	Přírodovědecká fakulta
Citace
www	https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.001743
Doi	http://dx.doi.org/10.1099/jgv.0.001743
Klíčová slova	biomarker; brain injury; flavivirus; neurofilament; tick-borne encephalitis
Popis	Extensive axonal and neuronal loss is the main cause of severe manifestations and poor outcomes in tick-borne encephalitis (TBE). Phosphorylated neurofilament heavy subunit (pNF-H) is an essential component of axons, and its detection in cerebrospinal fluid (CSF) or serum can indicate the degree of neuroaxonal damage. We examined the use of pNF-H as a biomarker of neuroaxonal injury in TBE. In 89?patients with acute TBE, we measured CSF levels of pNF-H and 3 other markers of brain injury (glial fibrillary acidic protein, S100B and ubiquitin C-terminal hydrolase L1) and compared the results to those for patients with meningitis of other aetiology and controls. Serum pNF-H levels were measured in 80?patients and compared with findings for 90 healthy blood donors. TBE patients had significantly (P<0.001) higher CSF pNF-H levels than controls as early as hospital admission. Serum pNF-H concentrations were significantly higher in samples from TBE patients collected at hospital discharge (P<0.0001) than in controls. TBE patients with the highest peak values of serum pNF-H, exceeding 10?000?pg?ml-1, had a very severe disease course, with coma or tetraplegia. Patients requiring intensive care had significantly higher serum pNF-H levels than other TBE patients (P<0.01). Elevated serum pNF-H values were also observed in patients with incomplete recovery (P<0.05). Peak serum pNF-H levels correlated positively with the duration of hospitalization (P=0.005). Measurement of pNF-H levels in TBE patients might be useful for assessing disease severity and determining prognosis.