Insight into peculiar adhesion of cells to plasma-chemically prepared multifunctional “amino-glue” surfaces

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Publikace nespadá pod Lékařskou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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BUCHTELOVÁ Martina BLAHOVÁ Lucie NEČAS David KŘÍŽKOVÁ Petra BARTOŠÍKOVÁ Jana MEDALOVÁ Jiřina KOLSKÁ Zdeňka HEGEMANN Dirk ZAJÍČKOVÁ Lenka

Rok publikování 2023
Druh Článek v odborném periodiku
Časopis / Zdroj Plasma Processes and Polymers
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www https://onlinelibrary.wiley.com/doi/10.1002/ppap.202200157
Doi http://dx.doi.org/10.1002/ppap.202200157
Klíčová slova amine plasma polymer; carboxyl plasma polymer; cell adhesion; glycocalyx; nonspecificadhesion
Popis Plasma polymers (PPs) can easily modify material surfaces to improve their bio-applicability due to match-made surface-free energy and functionality. However, cell adhesion to PPs typically composed of various functional groups has not yet been fully understood. We explain the origin of strong resistance to trypsin treatment previously noted for nonendothelial cells on amine PPs. It is caused mainly by nonspecific adhesion of negatively charged parts of transmembrane proteins to the positively charged amine PP surface, enabled by thin glycocalyx. However, endothelial cells are bound primarily by their thick, negatively charged glycocalyx and sporadically by integrins in kinetic traps, both cleaved by trypsin. Cell scratching by atomic force microscopy tip confirmed the correlation of trypsin resistance to the strength of cell adhesion.
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