The Role of Phosphatidylserine-positive Extracellular Vesicles in Intercellular Communication
| Autoři | |
|---|---|
| Rok publikování | 2023 |
| Druh | Další prezentace na konferencích |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Extracellular vesicles (EVs) are a heterogenous population of membrane vesicles derived from almost all cell types, even pathologically altered. Since they reflect the composition of their parental cells and are found in all body fluids, EVs are gaining attention by many researchers worldwide. While originally thought to function mainly in the elimination of cellular waste, EVs purpose is much more complex. EVs are essential mediators of intercellular communication and delivery particles of molecular signals. They substantially participate in maintaining homeostasis of healthy tissues and in progression of malignant disease transformation. Phosphatidylserine (PS) is a component of the plasma membrane, which occurs on the inner side of this membrane. PS is an important cell cycle signalling molecule, especially in relation to apoptosis. The exposure of PS on the outer surface of the membrane marks the apoptotic cell death. Furthermore, viable tumor cells often expose this phospholipid on their surface. Many studies also suggest that tumor cells release high amounts of EVs exposing PS at the surface. Accordingly, PS-EVs derived from tumor cells may be critically involved in the communication of tumor cells with other cells within the tumor microenvironment (TME). In this work, we isolated PS-EVs from head and neck squamous cell carcinoma (HNSCC) and cancer-associated fibroblasts (CAFs), characterised them and analysed their protein composition. The most represented proteins were found to be involved in processes, such as cell adhesion, and respectively, cell migration, apoptosis, angiogenesis, or intracellular signal transduction. As a next step, we observed the effect of these PS-EVs on normal fibroblasts (HGF) and hypopharyngeal carcinoma (FaDu cell line). After the influence of PS-EVs derived from CAF 101, FaDu cells showed resistance to apoptosis, thus EVs could serve as fundamental signal particles with high impact on tumor progression. |