The reverse tetracycline-controlled transactivator rtTA2s-S2 is toxic in mouse embryonic stem cells

Varování

Publikace nespadá pod Lékařskou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.

Autoři	BRYJA V. PACHERNÍK J. KUBALA L. HAMPL Aleš DVOŘÁK P.
Rok publikování	2003
Druh	Článek v odborném periodiku
Časopis / Zdroj	Reproduction, nutrition, development
Fakulta / Pracoviště MU	Přírodovědecká fakulta
Citace
Obor	Genetika a molekulární biologie
Klíčová slova	stem cells
Popis	The efficient and reversible control of transgene expression is a powerful tool for the correct manipulation of embryonic stem cells in both cell therapy and transgenesis. The aim of this work was to investigate the possibilities of recently developed reverse tetracycline-controlled transactivator rtTA2s-S2. We show that the rtTA2s-S2 is useful for transient inducible expression of genes in embryonic stem cells. However, we found that it was not possible to establish mouse embryonic stem cell lines stably expressing this transactivator. Using the viral IRES sequence which couples the expression of rtTA2s-S2 and neomycin phosphotransferase, we found that embryonic stem cells expressing rtTA2s-S2 are not capable of growing in the presence of G418. Our results indicate that this transactivator is toxic to ES cells and raise the need for the development of other strategies for stable and inducible expression of genes in ES cells.