Quantitative alterations of immunofluorescence staining for laminin-1 and matrix metalloproteinase-3 in spinal nerve roots of two neuropathic pain models
| Název česky | Kvantitativní změny imunofluorescenčního barvení lamininu 1 a matrix metaloproteinázy 3 v kořenech spinálního nervu u dvou modelů neuropatické bolesti |
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| Autoři | |
| Rok publikování | 2009 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Peripheral nerve injury leads to distal nerve stump degeneration. This process, which is called Wallerian degeneration, is accompanied by many cellular and molecular changes including myelin clearance and axonal breakdown. Events taking place in proximal nerve segments are less apparent and information about them in some compartments, such as spinal nerve roots, is very limited. Laminins, one group of extracellular matrix (ECM) molecules, are significantly involved in molecular processes related with regeneration of injured nerve. Matrix metalloproteinases are key enzymes influencing remodulation of extracellular matrix including laminins. We used quantitative immunohistochemical analysis of laminin-1 (lam-1) and matrix metalloproteinase-3 (MMP-3) in the spinal nerve roots following sciatic or spinal nerve ligature to confirm ECM alterations and its remodulation also more proximal to peripheral nerve injury. Compared to naive animals, a transient increase of lam-1 immunofluorescence (IF) intensity was observed in both ipsilateral and contralateral dorsal and ventral roots 1 and 3 days from sciatic or spinal nerve ligature. A bilateral decrease of lam-1 IF was detected in spinal nerve roots 7 or 14 days after both types of peripheral nerve injury. Brightness of MMP-3 IF gradually increased bilaterally in dorsal and ventral roots up to 3 (spinal nerve ligature) and 7 (sciatic nerve ligature) days from operation. A bilateral decrease of MMP-3 IF was found in roots during next monitored periods of survival - for spinal nerve ligature at 7 and for sciatic nerve ligature at 14 days following nerve injury. Alterations of IF were for both molecules generally more apparent in ipsilateral than contralateral roots. Larger changes of IF brightness were observed after sciatic than spinal nerve ligature. In conclusion, we demonstrated the remodulation of ECM in both ipsilateral and contralateral spinal nerve roots following unilateral peripheral nerve injury. In addition, we confirmed that matrix metalloproteinases are probably involved in this process. |
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