A real-time (PCR) for a real life ...? Quantitative evaluation of BCL2/IGH in follicular lymphoma and its implications for clinical practice

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Publikace nespadá pod Lékařskou fakultu, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.

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JANÍKOVÁ Andrea MAREČKOVÁ Andrea DVOŘÁKOVÁ Dana BORTLÍČEK Zbyněk TICHÝ Boris NAVRÁTIL Milan KRÁL Zdeněk POSPÍŠILOVÁ Šárka MAYER Jiří

Rok publikování 2012
Druh Článek v odborném periodiku
Časopis / Zdroj Experimental hematology
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://www.sciencedirect.com/science/article/pii/S0301472X12000847
Doi http://dx.doi.org/10.1016/j.exphem.2012.02.005
Obor Onkologie a hematologie
Klíčová slova POLYMERASE-CHAIN-REACTION; BONE-MARROW-TRANSPLANTATION; NON-HODGKINS-LYMPHOMA; CIRCULATING T(14-18)-POSITIVE CELLS; PREDICTS TREATMENT RESPONSE; T(14/18)-POSITIVE CELLS; PERIPHERAL-BLOOD; BCL-2/IGH REARRANGEMENTS; IMPROVED SURVIVAL; POSITIVE CELLS
Přiložené soubory
Popis Follicular lymphoma (FL) is highly associated with the molecular rearrangement BCL2/IGH. Although BCL2/IGH has been studied many times in follicular lymphoma, its real clinical value remains controversial. In this study, we performed quantitative testing by real-time polymerase chain reaction in 56 FL patients with median follow-up of 44 months (range, 9-102 months); chemotherapy was administered in 52 of 56 cases. Pretreatment numbers of BCL2/IGH varied in wide ranges, with a median of 2947 (range, 0-1,261,013) copies/10(6) cellular equivalent in peripheral blood (PB) and 4650 copies/10(6) cellular equivalent (range, 1-1,056,813) in bone marrow (BM), the difference between PB and BM was significant (p = 0.006). Pretreatment of BCL2/IGH quantities were correlated to clinical parameters (e.g., age, stage, sex, lactate dehydrogenase, B symptoms, grade, bulky disease, chemotherapy regimen) and to progression free-survival. Advanced clinical stage (III and IV) and microscopic BM involvement were significantly associated with higher numbers of BCL2/IGH in PB (p < 0.05) and in BM (p = 0.05), regardless all or newly diagnosed patients were evaluated. High pretreatment burden of BCL2/IGH was associated with significantly shorter progression-free survival; p = 0.003 and p = 0.047 for PB and BM, respectively. In conclusion, pretreatment quantity of BCL2/IGH in PB or BM seems to mirror the extent of disease and can provide an auxiliary prognostic parameter in FL. Our results also support evidence of the negative prognostic value of microscopic BM involvement in FL.
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