Publications from the yeast exoskeleton research

Kopecká, M., Phaff, H. J., Fleet, G. H. 1974. Demonstration of a fibrillar component in the cell wall of the yeast Saccharomyces cerevisiae and its chemical nature. J Cell Biol. 62:66-76. [IF=12.023]

Kopecká, M. 1984. Papulacandin B: inhibitor of biogenesis of 1,3-β-D-glucan fibrillar component of the cell wall of Saccharomyces cerevisiae protoplasts. Folia Microbiol. 29: 441-449. [IF=0.857]

Gabriel, M., Kopecká, M. 1995. Disruption of the actin cytoskeleton in budding yeast results in formation of an aberrant cell wall. Microbiology (UK) 141: 891-899. [IF=2.317]

Gabriel, M., Kopecká, M., Svoboda, A. 1992. Structural rearrangement of the actin cytoskeleton in regenerating protoplasts of budding yeast. J. Gen. Microbiol. 138: 2229-2234. [IF=2.034]

Kopecká, M., Gabriel, M. 1998. The aberrant positioning of nuclei and the microtubular cytoskeleton in Saccharomyces cerevisiae due to improper actin function. Microbiology (UK) 144: 1783-1797. [IF=2.564]

Kopecká, M. 1976. The use of antibiotic lomofungin for demonstration of nuclei and chromosomes in live yeast cells and protoplasts. Folia Microbiol. 21: 406-408. [IF=0.857]


ISHIGURO J., SHIMADA S., GABRIEL M., KOPECKÁ M. Characterization of a fission yeast mutant witch displays defects in cell wall integrity and cytokinesis. Genes Genet. Syst. 76: 257 - 269 (2001).

GABRIEL M., HORKÝ D., SVOBODA A., KOPECKÁ M. Cytochalasin D interferes with contractile actin ring and septum formation in Schizosaccharomyces japonicus var. versatilis. Microbiology (UK) 144: 2331-2344 (1998).

KOPECKÁ M. Yeast protoplasts – model for the study of mechanism of cell wall formation de novo (review article). Annual Report of Research Center for Pathogenic Fungi and Microbial Toxicoses Chiba University Japan 2: 47-57 (1998).

KOPECKÁ M., FLEET G. H., PHAFF H. J. Ultrastructure of the cell wall of Schizosaccharomyces pombe following treatment with various glucanases. J. Struct. Biol. 114: 104-152 (1995).

GABRIEL M., KOPECKÁ M. Morphogenesis of the cell wall in yeasts investigated in actin mutants and their protoplasts. In “Sixth Cell Wall Meeting“ Nijmegen. M. M. A. Sassen, J. W. M. Derksen, A. M. C. Emons, A. M. C. Wolters-Arts (eds). Nijmegen University Press, pp. 89-89 (1992).

KOPECKÁ M., GABRIEL M. Electron microscopy of the yeast cell wall. INVITED LECTURE. 8thInternational Symposium on Yeasts. Georgia State University, Atlanta, USA. Proceedings pp. 96-98 (1992). (S.A. Meyer, ed.)

KOPECKÁ M., GABRIEL M. The influence of Congo red on the cell wall and 1,3-ß-D-glucan microfibril biogenesis in Saccharomyces cerevisiae. Arch. Microbiol. 158: 115- 126 (1992).

KOPECKÁ M., GABRIEL M., NEČAS O., SVOBODA A., VENKOV P. V. Cell surface structures in osmotically fragile mutants of Saccharomyces cerevisiae. J. Gen. Microbiol. (UK) 137: 1263-1270 (1991).

ŠAJBIDOR J., KOPECKÁ M., ŠANDULA J., KAČURÁKOVÁ M. Lipids in yeast cell wall glucans. Microbios 68: 169-177 (1991).

SVOBODA A., TRUJILLO-GONZALES A.. Sporothrix schenckii - a freeze-fracture study. J. Basic Microbiol 30:371-378 (1990)

GABRIEL M., KOPECKÁ M. Studies on cell division in regenerating protoplasts of the yeast Schizosaccharomyces japonicus. J. Gen. Microbiol. (UK) 134: 2029-2034 (1988).

KOPECKÁ M., GABRIEL M., FARKAŠ V. Some anucleated yeast protoplasts synthesize ß-1,3-D-glucan microfibrils of the cell wall. Naturwissenschaften 74: 389-391 (1987).

KOPECKÁ M., KREGER D.R. Assembly of microfibrils in vivo and in vitro from 1,3-ß-D-glucan synthesized by protoplasts of Saccharomyces cerevisiae. Arch. Microbiol. 143:387-395 (1986).

KOPECKÁ M., GABRIEL M. Actin cortical cytoskeleton and cell wall synthesis in regenerating protoplasts of the Saccharomyces cerevisiae actin mutant DBY 1693. Microbiology (UK) 141: 1289-1299 (1995).

FARKAŠ V., HRMOVÁ M., KOPECKÁ M. Anucleated yeast protoplasts and their incapability to regenerate the cell wall. In: “Environmental Regulation of Microbial Metabolism“. I. S. Kulayev, E. A. Dawes, D. W. Tempest (eds.). Academic Press Inc., London pp. 491- 498 (1985).

KOPECKÁ M. Electron microscopic study of purified polysaccharide components glucans and mannan of the cell walls in the yeast Saccharomyces cerevisiae. J. Basic Microbiol. 25: 161-174 (1985).

GABRIEL M. Karyokinesis and septation in flattened Schizosaccharomyces versatilis protoplasts. Proceedings of International Symposium.”Physiology of Microbial Growth and Differentiation”. Reinhardsbrunn (1984).

GABRIEL M. Inhibition of the cell wall regeneration and reversion in protoplasts of Rhizopus nigricans. Folia Microbiol. 29: 365-374 (1984).

GABRIEL M. Karyokinesis and septum formation during the regeneration of incomplete cell walls in protoplasts of Schizosaccharomyces japonicus var. versatilis: a time-lapse microcinematographic study. J. Gen. Microbiol. (UK) 130: 625-630 (1984).

GABRIEL M. Formation, growth and regeneration of incomplete cell wall in spheroplasts of the blue-green alga Anacystis nidulans in liquid media. Z. Allgem. Mikrobiol. 24: 679-689 (1984).

HRMOVÁ M., FARKAŠ V., KOPECKÁ M. Isolation of anucleated yeast protoplasts by means of density gradient centrifugation. J. Microbiol. Methods (Elsevier) 2:257-263 (1984).

KOPECKÁ M. Lysis of growing cells of Saccharomyces cerevisiae induced by Papulacandin B. Folia Microbiol. 29: 115-11 (1984).

KOPECKÁ M., GABRIEL M. Ultrastructural study of incomplete cell wall enabling cell septum formation in regenerating Schizosaccharomyces versatilis protoplasts.ibid.(1984).

GABRIEL M. Karyokinesis and cell septum formation during incomplete cell wall regeneration in flattened Schizosaccharomyces versatilis protoplasts. ibid. pp. 144-146 (1983).

GABRIEL M., KOPECKÁ M. Regeneration of the cell wall of protoplasts of the fission yeast Schizosaccharomyces versatilis in liquid media and their reversion to cells. Folia Microbiol. 28: 452-457 (1983).

KOPECKÁ M. Formation of 1,3-ß-D-glucan fibrillar cell wall component in protoplasts from Hartwell´s cdc ts mutants blocked in various stages of Saccharomyces cerevisiae cell cycle. In: 6th European Cell Cycle Workshop“Progress in Cell Cycle Controls“. Prague. J. Chaloupka, A. Kotyk, E. Streiblová (eds.). Institute of Microbiology Czechoslovak Academy of Sciences, pp. 166-168 (1983).

KREGER D.R., KOPECKÁ M. The molecular organization of chitin in normal and regenerated walls of Saccharomyces cerevisiae. A reconsideration of ultrastructural data. In: “Cell Walls” 1981. Proceedings of the Second Cell Wall Meeting Göttingen. D. G. Robinson, H. Quader (eds.). Wissenschaftliche Verlagsgesellschaft mbH Stuttgart, pp.130-134 (1981).

GABRIEL M. Vlijanie častičnoj experimentalnoj blokady syntěza stěnki protoplastov pleseni Rhizopuis nigricans na kletočnyj morfogeněz. Scripta medica (Brno) 53: 333-336 (1980).

NEČAS O., GABRIEL M. Ultrastructure of spores of Rhizopus nigricans after repeated freezing and thawing shocks. Folia Microbiol. 25: 228-232 (1980).

KOPECKÁ M., FARKAŠ V. RNA synthesis and the formation of the cell wall. Effect of lomofungin on regenerating protoplasts of Saccharomyces cerevisiae. J. Gen. Microbiol. (UK) 110: 453-463 (1979).

KOPECKÁ M., GABRIEL M. Staining the nuclei in cells and protoplasts of living yeasts, moulds and green algae with the antibiotic lomofungin. Arch. Microbiol. 119: 305-311 (1978).

KREGER D.R., KOPECKÁ M. Nature of the nets produced by protoplasts of Schizosaccharomyces pombe during the first stage of wall regeneration in liquid media. J. Gen. Microbiol. (UK) 108: 269-274 (1978).

GABRIEL M., HAVELKOVÁ M., KOPECKÁ M., SVOBODA A. Cell wall regeneration in protoplasts as a model for the study of biogenesis of cell organelle. Scripta medica (Brno) 59: 29-34 (1977).

KOPECKÁ M. The use of antibiotic lomofungin for demonstration of nuclei and chromosomes in live yeast cells and protoplasts. Folia Microbiol. 21:406 - 408 (1976).

KREGER D.R., KOPECKÁ M. On the nature and formation of the fibrillar nets produced by protoplasts of Saccharomyces cerevisiae in liquid media: an electronmicroscopic, X-ray diffration and chemical study. J. Gen. Microbiol (UK) 92:207-220 (1976).

KREGER D. R., KOPECKÁ M. Assembly of wall polymers during the regeneration of yeast proto-plasts. In: “Microbial and Plant Protoplasts”. J. F. Peberdy, A. H. Rose, H. J. Rogers, E. C. Cocking (eds.). Academic Press London, New York, San Francisco, pp. 237-252 (1976).

KOPECKÁ M. The isolation of protoplasts of the fission yeast Schizosaccharomyces by Trichoderma viridae and snail enzymes. Folia Microbiol. 20:273-276 (1975).

GABRIEL M. Cell wall regeneration and reversion in protoplasts of green alga, Uronema gigas. Proceedings of the Fourth International Symposium on Yeasts. Vienna, Austria. 1974. H. Klaushofer and U. B. Sleytr (eds.) pp. 195-196 (1974).

KOPECKÁ M. Sporulation in protoplasts of the yeast, Saccharomyces cerevisiae. J. Gen Microbiol. (UK) 83:171-178 (1974).

KOPECKÁ M., GABRIEL M., NEČAS O. A method of isolating anucleated yeast protoplasts unable to synthesize the glucan fibrillar component of the wall. J. Gen. Microbiol. (UK) 81: 111-120 (1974).

KOPECKÁ M., PHAFF H. J. On the ultrastructure of the yeast cell wall. Biológia (Bratislava) 29:927-936 (1974).

KOPECKÁ M., PHAFF H.J., FLEET G.H. The ultrastructure of the yeast cell wall after enzymic degradation of by purified enzymes. Proceedings of the Fourth International Symposium on Yeasts. Vienna, Austria. 1974. H. Klaushofer and U.B. Sleytr (eds.) pp. 205-206 (1974).

KOPECKÁ M., SVOBODA A., BRICHTA J. Effect of „osmotic stabilizers“ and glycerol on yeast cell envelopes. Z. Allg. Microbiol. 13: 481-48 (1973).

KREGER D. R., KOPECKÁ M. On the nature of the fibrillar nets formed by protoplasts of Saccharomyces cerevisiae in liquid media. In: “Yeast, Mould and Plant Protoplasts”. J. R. Villanueva, I. Garcia-Acha, S. Gascon, F. Uruburu (eds.). Academic Press London and New York, pp. 117-130 (1973).

KOPECKÁ M. Dictyosomes in the yeast Schizosaccharomyces pombe. Ant. van Leeuwenhoek 38: 27-31(1972).

GABRIEL M. Formation and growth of protoplasts of alga Uronema gigas. Proceedings of the 2nd International Symposium on Yeast Protoplasts. Brno. 1968. (O. Nečas and A. Svoboda, eds.). Acta Fac. Med. Univ. Brno 37: 153-157 (1970).

GABRIEL M. Cell wall regeneration in Rhizopus nigricans protoplasts. ibid. pp. 147-151 (1970).

GABRIEL M. Formation, growth and regeneration of protoplasts of the green alga, Uronema gigas. Protoplasma 70:135-138 (1970).

KOPECKÁ M., NEČAS O., SVOBODA A. Structure and formation of the fibrillar component of the growing wall in yeast protoplasts. ibid. pp. 197-201 (1970).

NEČAS O., KOPECKÁ M., BRICHTA J. Surface structures of yeast protoplasts as revealed by freeze-etching. ibid. pp. 33-38 (1970).

NEČAS O., SOŠKA J., KOPECKÁ M., REICH J. Application of 3H-glucose in the study of cell wall synthesis in yeast protoplasts. ibid. pp. 203-209 (1970).

KOPECKÁ M., SVOBODA A., NEČAS O. Action of ribonuclease on growth, synthesis of cell wall and ultrastructure in yeast protoplasts and cells. Ant. van Leeuwenhoek 35: Suppl. B9-B10 (1969).

NEČAS O., KOPECKÁ M. Synthesis of the fibrillar component of regenerating cell walls in yeast protoplasts. Ant. van Leeuwenhoek 35: Suppl. B7-B8 (1969).

NEČAS O., KOPECKÁ M., BRICHTA J. Interpretation of surface structures in frozen-etched protoplasts of yeasts. Exp. Cell Res . 58: 411-419 (1969).

GABRIEL M. Formation and regeneration of protoplasts in the mould Rhizopus nigricans. Folia Microbiol. 13: 231-234 (1968).

NEČAS O., SVOBODA A., KOPECKÁ M. The effect of cycloheximide (actidione) on cell wall synthesis in yeast protoplasts. Exp. Cell Res. 53:291-293 (1968).

GABRIEL M. Vergleichende Untersuchungen der Regeneration von Pilz- und Hefeprotoplasten. Symposium über Hefe-Protoplasten Jena, 1965. (R. Müller, ed.). Akademie Verlag Berlin, pp. 217-222, 233-235 (1967).

KOPECKÁ M., ČTVRTNÍČEK O., NEČAS O. Formation and properties of the fibrillar network formed in yeast protoplasts as the first step of biosynthesis of cell wall. ibid. pp.73-75, 343-344 (1967).

NEČAS O., GABRIEL M. Regenerace buněk nižších rostlin. In:“Regenerace buňky“ (O. Nečas, ed.). Acta Fac. Med. Univ. Brno 14: 66-110 (1965).

NEČAS O., JANISCH R., JAHODA J., GABRIEL M. Division of the nuclei of naked yeast protoplasts. Folia biol. 7: 202-205 (1961).

prof. MUDr. Marie Kopecká, CSc.

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The cytoskeleton in human pathogenic fungi