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Would you like to understand the prenatal programming of brain development?
Do you have a master's degree in Mathematical biology and biomedicine, Bioinformatics, Psychology, or a related field?
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Multi-modal and Functional Neuroimaging Research Group is
Opening new PhD positions
in the field of Neurosciences
A unique research cluster for the treatment of stroke has been established in Brno
STROKE BRNO is an interdisciplinary research cluster with the aim of connecting the knowledge and expertise of academic and industrial partners and ensuring the effective use of knowledge from basic research in clinical practice.
Our Latest Research
Relative Entropy is an easy-to-use invasive EEG Biomarker of the Epileptogenic Zone
Objective: High-frequency oscillations are considered among the most promising interictal biomarkers of the epileptogenic zone in patients suffering from pharmaco-resistant focal epilepsy. However, there is no clear definition of pathological High-frequency oscillations and the existing detectors vary in methodology, performance, and computational costs. This study proposes Relative Entropy as an easy-to-use novel interictal biomarker of the epileptic tissue.
Methods: We evaluated Relative Entropy and High-frequency oscillations biomarkers on intracranial EEG data from 39 patients with seizure-free postoperative outcome (Engel Ia) from three Institutions. We tested their capability to localize the epileptogenic zone, defined as resected contacts located in seizure-onset zone. The performance was compared using areas under the receiver operating curves [AUROC] and precision-recall curves [AUPRC].
Basilar artery occlusion management: Specialist perspectives from an international survey
Background and purpose: Two early basilar artery occlusion (BAO) randomized controlled trials did not establish the superiority of endovascular thrombectomy (EVT) over medical management. While many providers continue to recommend EVT for acute BAO, perceptions of equipoise in randomizing patients with BAO to EVT versus medical management may differ between clinician specialties.
Methods: We conducted an international survey (January 18, 2022 to March 31, 2022) regarding management strategies in acute BAO prior to the announcement of two trials indicating the superiority of EVT, and compared responses between interventionalists (INTs) and non-interventionalists (nINTs). Selection practices for routine EVT and perceptions of equipoise regarding randomizing to medical management based on neuroimaging and clinical features were compared between the two groups using descriptive statistics.
Parental education, cognition and functional connectivity of the salience network
The aim was to investigate the association of parental education at birth with cognitive ability in childhood and young adulthood and determine, whether functional connectivity of the salience network underlies this association. We studied participants of the Czech arm of the European Longitudinal Study of Pregnancy and Childhood who underwent assessment of their cognitive ability at age 8 (Wechsler Intelligence Scale for Children) and 28/29 years (Wechsler Adult Intelligence Scale) and measurement with resting state functional MRI at age 23/24. We estimated the associations of parental education with cognitive ability and functional connectivity between the seeds in the salience network and other voxels in the brain. We found that lower education of both mothers and fathers was associated with lower verbal IQ, performance IQ and full-scale IQ of the offspring at age 8. Only mother´s education was associated with performance IQ at age 28/29.
Association of statin pre-treatment with baseline stroke severity and outcome in patients with acute ischemic stroke and received reperfusion treatment: An observational study
Background: Statins have an important role in stroke prevention, especially in high-risk populations and may also affect the initial stroke severity and outcomes in patients taking them before an ischemic stroke.
Aims: Our aim was to evaluate the association of statin pre-treatment with the severity in acute ischemic stroke (AIS).
Methods: We analyzed AIS patients received intravenous thrombolysis (IVT) and/or endovascular thrombectomy (EVT) and recorded in the SITS International Thrombolysis and Thrombectomy Registry from 2011 to 2017. We identified patients with statin information at baseline. The primary outcome was baseline National Institutes of Health Stroke Scale (NIHSS) score.
Stroke Learning Health Systems: A Topical Narrative Review With Case Examples
To our knowledge, the adoption of Learning Health System (LHS) concepts or approaches for improving stroke care, patient outcomes, and value have not previously been summarized. This topical review provides a summary of the published evidence about LHSs applied to stroke, and case examples applied to different aspects of stroke care from high and low-to-middle income countries. Our attempt to systematically identify the relevant literature and obtain real-world examples demonstrated the dissemination gaps, the lack of learning and action for many of the related LHS concepts across the continuum of care but also elucidated the opportunity for continued dialogue on how to study and scale LHS advances. In the field of stroke, we found only a few published examples of LHSs and health systems globally implementing some selected LHS concepts, but the term is not common. A major barrier to identifying relevant LHS examples in stroke may be the lack of an agreed taxonomy or terminology for classification.
Single Cerebral Organoid Mass Spectrometry of Cell-Specific Protein and Glycosphingolipid Traits
Cerebral organoids are a prolific research topic and an emerging model system for neurological diseases in human neurobiology. However, the batch-to-batch reproducibility of current cultivation protocols is challenging and thus requires a high-throughput methodology to comprehensively characterize cerebral organoid cytoarchitecture and neural development. We report a mass spectrometry-based protocol to quantify neural tissue cell markers, cell surface lipids, and housekeeping proteins in a single organoid. Profiled traits probe the development of neural stem cells, radial glial cells, neurons, and astrocytes. We assessed the cell population heterogeneity in individually profiled organoids in the early and late neurogenesis stages. Here, we present a unifying view of cell-type specificity of profiled protein and lipid traits in neural tissue. Our workflow characterizes the cytoarchitecture, differentiation stage, and batch cultivation variation on an individual cerebral organoid level.
International study: Global impact of COVID-19 on stroke care - the Polish contribution
The most severe consequences of the COVID-19 pandemic were reported during its first wave in 2019. Although airway inflammation is part of the main clinical picture of COVID-19, the consequences of a SARS-Cov2 infection can also include coagulation disorders, cardiomyopathy and endotheliopathy, which lead to stroke in 1.5% of patients.
With the global reach of the pandemic came collaboration between scientists and clinicians from around the world.
This transfer of knowledge between various medical centres contributed to greatly improved understanding of the mechanisms of transmission and the different manifestations of infection.
To assess the global impact of the pandemic on stroke hospitalisations and outcomes, Prof. Nogueira inspired the international community of neurologists and neurointerventionalists from six continents, 70 countries and almost 500 hospital stroke centres to participate in large, cross-sectional, observational, retrospective studies
A preliminary choroid plexus volumetric study in individuals with psychosis
The choroid plexus (ChP) is part of the blood-cerebrospinal fluid barrier, regulating brain homeostasis and the brain's response to peripheral events. Its upregulation and enlargement are considered essential in psychosis. However, the timing of the ChP enlargement has not been established. This study introduces a novel magnetic resonance imaging-based segmentation method to examine ChP volumes in two cohorts of individuals with psychosis. The first sample consists of 41 individuals with early course psychosis (mean duration of illness = 1.78 years) and 30 healthy individuals. The second sample consists of 30 individuals with chronic psychosis (mean duration of illness = 7.96 years) and 34 healthy individuals. We utilized manual segmentation to measure ChP volumes. We applied ANCOVAs to compare normalized ChP volumes between groups and partial correlations to investigate the relationship between ChP, LV volumes, and clinical characteristics. Our segmentation demonstrated good reliability (.87).