EFFECT OF PLATELET-RICH PLASMA, PLATELET-RICH FIBRIN AND ENAMEL MATRIX DERIVATIVE ON SOFT TISSUE HEALING. EXPERIMENTAL STUDY IN RABBITS
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| Year of publication | 2015 |
| Type | Conference abstract |
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| Description | Introduction: Regenerative medicine aims to enable and promote reparation or regeneration of tissues. In dentistry, many different approaches are tested or already used to enhance healing of either hard or soft tissues, including use of cytokines and growth factors (GF) to modify cellular behaviour during healing. Growth factors are found in large numbers in autologous blood concentrates – platelet-rich plasma (PRP) and platelet-rich fibrin (PRF). Enamel matrix derivative (EMD) extracted from porcine fetuses may improve tissue healing via amelogenins and other enamel matrix proteins. Bilayered collagen matrix (CM) is used for soft tissue augmentation in periodontology and implant dentistry and might be an alternative to palatal connective tissue graft (CTG) in recession coverage. Objectives of the investigation: The aim of the presented work is to evaluate the effect of blood plasma derivatives (PRP, PRF) or EMD added to CM during soft tissue regeneration in a split-mouth prospective study. Experimental methods used: Twenty-seven rabbits were included in this trial. To each one of them 5 artificial defects of diameter 6 mm and depth 1-2 mm were made in the palatal and maxillary region of oral mucosa. Subsequently, defects were treated with PRP+CM, PRF+CM, EMD+CM, CM only or left untreated as a negative control (CO). Animals were euthanized 1 day, 7 days and 28 days after surgery and necropsies were harvested. Histological analysis assessed acute and chronic inflammatory infiltrate, level of angiogenesis and re-epithelialization, quantity of collagen, macrophages and granulation tissue. These parameters were rated semiquantitatively on 0-3 scale for each specimen, newly formed vessels were counted in high-power field. Essential results including data, and eventual statistics: By the day 28 all of the wounds have been closed and completely re-epithelialized. No adverse reaction has been observed. No difference has been shown by PRP+CM, PRF+CM, CM and CO group in any time point. Slower angiogenesis and granulation tissue formation was revealed in EMD+CM group 1 day and 7 days postoperatively. This difference reached statistical significance. Conclusion: We did not prove any clinical advantage of combined use of CM as a carrier of PRP, PRF or EMD when treating mucosal wounds in animal model. These results are to be confirmed in following studies. |
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