Detection of selected periodontal bacteria in preschool children affected by early childhood caries

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Authors

PANTUČKOVÁ Pavla BARTOŠOVÁ Michaela BROUKAL Zdeněk KUKLETOVÁ Martina IZAKOVIČOVÁ HOLLÁ Lydie

Year of publication 2016
Type Article in Periodical
Magazine / Source Folia Microbiologica
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.1007/s12223-016-0468-5
Field ORL, ophthalmology, stomatology
Keywords PORPHYROMONAS-GINGIVALIS; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; SUBGINGIVAL MICROBIOME; HEALTHY-CHILDREN; MIXED DENTITION; ORAL MICROBIOTA; YOUNG-CHILDREN; PRIMARY TEETH; COLONIZATION; PATHOGENS
Description The aim of this study was to compare the detection frequency of periodontal bacteria in dental plaque in children with early childhood caries (ECC) with and without gingival inflammation. A convenience sample of 25 preschool children (mean age 3.61 years, SD 1.42) was recruited. Dental plaque was taken from periodontal areas with and without visible signs of inflammation and processed using the StomaGeneA (R) (Protean s.r.o. Czech Republic) and ParoCheckA (R) 20 (Greiner Bio-one GmbH, Germany) detection kits. The two sample t tests between percents for differences between inflammatory and healthy sites and kappa statistics for the agreement of both systems were used. At the inflammatory sites, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans were significantly more frequently detected by StomaGeneA (R) while Fusobacterium nucleatum, A. actinomycetemcomitans, Tanarella forsythia and Prevotella intermedia were significantly more frequently identified by ParoCheckA (R) test. The agreement between the two detection systems was substantial for A. actinomycetemcomitans and F. nucleatum in the samples collected from inflamed sites and only for F. nucleatum from clinically healthy sites. Therefore, we recommend that the same system should be used when the same patient is examined repeatedly.
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