Jednoduchá metoda k průkazu CD154 (CD40L) na lymfocytech v periferní krvi
| Title in English | A simple method for the detection of CD154 (CD40L) on peripheral blood lymphocytes |
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| Authors | |
| Year of publication | 2010 |
| Type | Article in Periodical |
| Magazine / Source | Epidemiologie Mikrobiologie Imunologie |
| MU Faculty or unit | |
| Citation | |
| Field | Immunology |
| Keywords | CD154; CD40L; CD40; hyper-IgM syndrome; flow cytometry |
| Description | CD154 (also called CD40L) is a transmembrane glycoprotein predominantly expressed on the surface membrane of activated CD4+ T cells. Its receptor CD40 is present on all B cells, but also on other cells. The interaction CD154-CD40 is necessary for the optimal development of the adaptive immune response and also has consequences for the modulation of the inflammatory response. A defect in the expression of CD154 is pathognomonic of congenital immunodeficiency called X-linked Hyper-IgM syndrome (XHIGM). To detect the abnormality of CD154 is essential for making the diagnosis of XHIGM. The presented microtest for the detection of CD154 on activated lymphocytes in whole blood is fast and blood saving, since as little as 0.5 ml of blood is needed to complete it. It can be recommended as the initial test for suspected hyper-IgM syndrome in children. We demonstrate that this screening method can help to detect also carriers of XHIGM. |
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