WNT unrelated activities in commercially available preparations of recombinant WNT3a


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Title in English WNT unrelated activities in commercially available preparations of recombinant WNT3

ČAJÁNEK Lukáš ADLERZ Linda BRYJA Vítězslav ARENAS Ernest

Year of publication 2010
Type Article in Periodical
Magazine / Source Journal of Cellular Biochemistry
MU Faculty or unit

Faculty of Science

Field Genetics and molecular biology
Keywords WNT pathway; ligand; purification; signaling; AKT/GSK3; phosphorylation; SFRP1; beta-catenin
Description WNT signaling pathways play an important role in both development and disease. By analyzing the signaling capabilities of commercially available WNT3a preparations towards the PI3K/AKT/GSK3 signaling pathway, we discovered unexpected inconsistencies from lot to lot of recombinant WNT3a. We provide evidence that: (1) The ability to trigger AKT/GSK3 signaling varies dramatically between different lots of WNT3a, without any variation in their ability to activate the canonical WNT/beta-catenin signaling. (2) sFRP1, a WNT signaling inhibitor, is unable to interfere with the activation of AKT/GSK3 signaling induced by some of the WNT3a lots. (3) Pharmacological inhibition of AKT/GSK3 phosphorylation by PI3K inhibitors fails to affect the stabilization of beta-catenin, the central effector of the canonical WNT/beta-catenin signaling pathway.
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