WNT unrelated activities in commercially available preparations of recombinant WNT3a

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Title in English WNT unrelated activities in commercially available preparations of recombinant WNT3
Authors

ČAJÁNEK Lukáš ADLERZ Linda BRYJA Vítězslav ARENAS Ernest

Year of publication 2010
Type Article in Periodical
Magazine / Source Journal of Cellular Biochemistry
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords WNT pathway; ligand; purification; signaling; AKT/GSK3; phosphorylation; SFRP1; beta-catenin
Description WNT signaling pathways play an important role in both development and disease. By analyzing the signaling capabilities of commercially available WNT3a preparations towards the PI3K/AKT/GSK3 signaling pathway, we discovered unexpected inconsistencies from lot to lot of recombinant WNT3a. We provide evidence that: (1) The ability to trigger AKT/GSK3 signaling varies dramatically between different lots of WNT3a, without any variation in their ability to activate the canonical WNT/beta-catenin signaling. (2) sFRP1, a WNT signaling inhibitor, is unable to interfere with the activation of AKT/GSK3 signaling induced by some of the WNT3a lots. (3) Pharmacological inhibition of AKT/GSK3 phosphorylation by PI3K inhibitors fails to affect the stabilization of beta-catenin, the central effector of the canonical WNT/beta-catenin signaling pathway.
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