Assessment of the Cronobacter sakazakii risk in reconstituted infant formula
| Authors | |
|---|---|
| Year of publication | 2025 |
| Type | Article in Periodical |
| Magazine / Source | Acta Veterinaria Brno |
| MU Faculty or unit | |
| Citation | |
| web | https://actavet.vfu.cz/94/1/0067/ |
| Doi | https://doi.org/10.2754/avb202594010067 |
| Keywords | Predictive microbiology; Baranyi-Roberts model; lag phase; growth rate |
| Attached files | |
| Description | The study focused on assessing Cronobacter sakazakii growth in reconstituted powdered infant formula at temperatures ranging from 5 degrees C to 48 degrees C using the Baranyi-Roberts model. The count of C. sakazakii was determined by the plate method on ESIA agar (44 degrees C, 24 h). Bacteria grew in reconstituted milk only at temperatures above 8 degrees C. The lag phase duration decreased with increasing temperature, from approximately 123.0-141.0 h at 8 degrees C to 0.931-1.281 h at 44 degrees C. The growth rate ranged from 0.025-0.027 ln cfu/ml/h (8 degrees C) to 2.441-2.633 ln cfu/ml/h (44 degrees C). The resulting growth models imply an increase of more than 4 orders of magnitude in the number of C. sakazakii in less than 17 h at 24 degrees C; at temperatures of 27 degrees C and above, the bacteria reach the critical concentration considered in our study (8 log cfu) in a few hours (4.5-11.9 h). In conclusion, it is unsafe and inappropriate to store reconstituted infant milk at temperatures higher than 8 degrees C. |