Bakteriocín kmeňa Yersinia frederiksenii 27601

Title in English Bacteriocin of strain Yersinia frederiksenii 27601


Year of publication 2009
Type Article in Proceedings
Conference XIII. Setkání biochemiků a molekulárních biologů. Sborník příspěvků.
MU Faculty or unit

Faculty of Medicine

Field Microbiology, virology
Keywords bacteriocin; Yersinia; colicin F
Description In a cross-test experiment, we investigated the ability of 53 nonpathogenic strains of the genus Yersinia to produce antibacterial agents. We identified six bacteriocin producers. Bacteriocin of Y. fredericksenii 27601 was further analyzed. The identified bacteriocin of Y. fredericksenii 27601 is a protein with specific antibacterial activity on bacteria belonging to the related strains of the genus Yersinia. Bacteriocin and immunity protein encoding genes were identified on the plasmid pYF27601. Plasmid pYF27601 showed high degree of homology to Col-plasmids and its sequencing revealed gene encoding bacteriocin (1317 bp) and immunity protein gene (338 bp). Sequence analysis of the bacteriocin gene revealed homology to genes of alveicin A and alveicin B, colicins Ia and Ib, S-type pyocin and colicin E1. The above described bacteriocin of Y. fredericksenii is a new type of bacteriocin. We propose to denominate it as colicin FY.
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