Analýza fenolických kyselin artyčoku pomocí vysokoúčinné kapalinové chromatografie s coulometrickou detekcí
| Title in English | Analytical procedure for determination of phenolic acids in artichoke (Cynara spp.) using HPLC with coulometric detection |
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| Authors | |
| Year of publication | 2001 |
| Type | Article in Proceedings |
| Conference | Biologicky aktivní fenolické látky v zemědělských plodinách |
| MU Faculty or unit | |
| Citation | |
| Field | Analytic chemistry |
| Keywords | Cynara; HPLC; phenolic acids; electrochemical detection |
| Description | Analysis of artichoke phenolic acids using reversed-phase high-performance liquid chromatography with coulometric detection is described. The HPLC system consisted of an LiChrospher 100 RP-18 column (5 um, 125 x 4 mm) and a mobile phase with 35% methanol in 50 mM phosphate buffer (pH 2.5). High selectivity and a low detection limits were achieved at a low potential (0-150 mV). The method was applied to identification of caffeoylquinic acids metabolites in human urine after a single dose of an extract from the leaves of Cynara cardunculus taken orally. |
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