Identification of Quantum Dots Labeled Metallothionein by Fast Scanning Laser-Induced Breakdown Spectroscopy

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Authors	KONEČNÁ Marie NOVOTNÝ Karel KŘÍŽKOVÁ Soňa BLAŽKOVÁ Iva KOPEL Pavel KAISER Jozef HODEK Petr KIZEK René ADAM Vojtěch
Year of publication	2013
Type	Conference abstract
MU Faculty or unit	Central European Institute of Technology
Citation
Description	LIBS was applied for rapid determination of cadmium presented in Cd-containing QDs (CdS, CdTe), which were used for labeling of MT, anti-MT and anti-Cd antibodies. Metallothionein and both antibodies were deposited on the polystyrene surface. 2D fast scanning was used for detection of QDs labeled proteins. The detection was based on the quantification of cadmium on the emission line 508.58 nm. In order to reach high sensitivity and high spatial resolution single pulse and double pulse technique was optimized and several types of raster and line scanning modes were used. Due to the application of Nd:YAG lasers UP-266 MACRO, Brilliant and detector PI Max3 repetition rate up to 10 Hz was used for fast scanning and 2D mapping of spatial distribution cadmium on the polystyrene surface.
Related projects:	CEITEC - Central European Institute of Technology