Time-resolved study of microorganisms by Raman spectroscopy

Authors

SAMEK Ota HARONIKOVA Andrea OBRUCA Stanislav BERNATOVA Silvie JEZEK Jan SILER Martin MLYNÁRIKOVÁ Katarína ZEMANEK Pavel

Year of publication 2015
Type Article in Proceedings
Conference Clinical and Biomedical Spectroscopy and Imaging IV
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.1117/12.2183950
Field Medical equipment
Keywords Raman spectroscopy; time-resolved study; red yeast; Cupriavidus necator H16
Description The main goal of our investigations is to focus on the basic physiological mechanisms of microorganisms (yeast and bacteria), exposed to different conditions, by time-resolved Raman spectroscopy. This study provides an insight into the mechanism of targeted stress factors or the influence of different cultivation times on species metabolism in vivo, in real-time and label free. We also focused on time-course study of physico-chemical properties of bacterial cells and cell cytoplasm with respect to the intracellular content of polyhydroxyalkanoates and to the production of yeast lipids or carotenoids.

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