DNA and Chromatin Structure of Telomere-Subtelomere Junctions in Silene latifolia

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Authors

SÝKOROVÁ Eva HORÁKOVÁ Mirka MIKAKO I. FUKUI K. FAJKUS Jiří

Year of publication 2000
Type Article in Periodical
Magazine / Source J.Biomol. Struct. Dyn.
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords Telomere; Subtelomere; Chromatin; Silene latifolia
Description Genomic variants of telomere-subtelomere junctions in Silene latifolia chromosomes were cloned and sequenced. In addition to the previously isolated subtelomeric sequence X43.1 (Buzek et al., 1997), several novel telomere-associated sequences have been characterised, including high-copy tandem repeat 15Ssp and low-copy sequence BstB19. Their subtelomeric location has been confirmed by pulsed-field gel electrophoresis and fluorescent in situ hybridisation. Analysis of chromatin structures (nucleosome phasing, nucleosome positioning) at these sequences revealed their specific features
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