Metalothionein - cisplatina a protirakovinová terapie.
| Title in English | Metallothionein -- cisplatine and anticancer therapy |
|---|---|
| Authors | |
| Year of publication | 2004 |
| Type | Article in Periodical |
| Magazine / Source | Klinická biochemie a metabolismus |
| MU Faculty or unit | |
| Citation | |
| Field | Electrochemistry |
| Keywords | analysis of MT; carcenogenisis; cisplatine; gene therapy; heavy metals; metallothionein |
| Description | Metallothioneins are low-molecular proteins which are taking part on supervision of metal homeostase. About molecular mechanisms of MT expression it is known too little and probably single metal is participating in MT expression by bonding on transcriptional factor MTF-1. Recently researches denote at important relatives between MT concentration and carcenogenesis, mutagenesis and teratogens. MT is known as prognostic marker at many kinds of cancer and contain an affinity to anticancer drugs. For example cisplatine is used more than 40 years and is engages to quanin in DNA molecule. However cell can recognise creating adduct and by correcting mechanisms aspires to repair it. Rising of metal concentration in organism leads to increasing MT expression and subsequently healthy effect is markedly reduced. Using of antisense mRNA, genes therapy, protease and application another metals is an option how we can defend organisms to the contrary of these unadvisable effects. Necessary condition of study of behaviour biological molecules are good applicable analytical methods. Methods using for MT determination are beyond electrophoresis and chromatography, also electrochemistry or mass spectrometry. |