Synergismus antibiotik u bakteriálního biofilmu
| Title in English | Synergy testing in bacterial biofilm |
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| Authors | |
| Year of publication | 2004 |
| Type | Article in Proceedings |
| Conference | Bulletin Československé společnosti mikrobiologické |
| MU Faculty or unit | |
| Citation | |
| Field | Microbiology, virology |
| Keywords | biofilm - antibiotic |
| Description | Many bacteria are able to form a biofilm. Bacteria in biofilm are less susceptible to antibiotics. After biofilm detection with modified Christensen method, we prepared 24-hour old biofilm in trypticase soy broth medium on 96-well polystyren microtiter plates. We tested antibiotics individually and in combinations. Minimum biofilm inhibitory concentration (MBIC) and fractionate inhibitory concentration (FBIC) were determined. We detected 34 biofilm forming strains (37%) out of 92 tested strains, who were Gram-negative as well as Gram-positive. Bacteria in biofilm were 8x to 256x resistant than planctonic growing bacteria. Synergy was demonstrated although the strains were resistant to the individual antibiotics. We demonstrated synergism or partial synergism against 32.4 % of isolates with one or more antimicrobial combinations. |
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