Actin in Malassezia pachydermatis detected by immunogold labeling for electron microscopy
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| Year of publication | 2006 |
| Type | Conference abstract |
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| Description | M. pachydermatis is lipophilic yeast that can be the cause of various diseases of animals (otitis externa, dermatitis) and humans (sepsis). The cells have a compact, relatively thick wall, a unique spiral pattern of invaginations of the plasma membrane, a ring-like swelling at the broad budding base and circumvallate bulgings near the budding base. The cytoskeletal studies of this species by fluorescent microscopy are complicated due to a small size of its cells and their agglutination. Because the detection of actin with rhodamine-phalloidin and imunofluorescence failed, we attempted to demonstrate this cytoskeletal protein in ultrathin sections by electron microscopy, using antibodies conjugated with gold particles. We observed accumulation of gold particles under the plasma membrane near the invaginations. This implies that actin can be located to submembrane regions of these cells. This work was supported by Grant Agency of the Czech Republic (grants 310/03/1195,310/06/0605 and 301/03/H005). |
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