Cytoskeletal structures, ultrastructural characteristics and the capsule of the basidiomycetous yeast Cryptococcus laurentii
| Authors | |
|---|---|
| Year of publication | 2007 |
| Type | Article in Periodical |
| Magazine / Source | Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology |
| MU Faculty or unit | |
| Citation | |
| Web | |
| Field | Other medical specializations |
| Keywords | capsule; cell cycle; Cryptococcus laurentii; cytoskeleton; morphology; ultrastructure |
| Description | The cytoskeleton, capsule and cell ultrastructure were studied during the cell cycle of Cryptococcus laurentii. In an encapsulated strain, cytoplasmic microtubules and a mitotic spindle were detected. Mitosis was preceded by migration of the nucleus into the bud. F-actin failed to be visualised by rhodamine-phalloidin (RhPh) in encapsulated cells and therefore an acapsular strain was used. The following actin structures were found: actin dots, actin cables and cytokinetic ring. Ultrastructural studies showed the presence of a nucleus in the bud before mitosis. A collar-shaped structure was seen at the base of bud emergence. A lamellar cell wall and a rough outer surface of the cells were detected. Cytoskeletal structures found in Cryptococcus laurentii are similar to those in Cryptococcus neoformans, which is a serious human pathogen. |
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