Low-frequency magnetic field effect on cytoskeleton and chromatin
| Authors | |
|---|---|
| Year of publication | 2007 |
| Type | Article in Periodical |
| Magazine / Source | Bioelectrochemistry |
| MU Faculty or unit | |
| Citation | |
| Field | Biophysics |
| Keywords | ELF; low-frequency magnetic field; cytosceleton; chromatin; apoptosis; |
| Description | We studied low-frequency magnetic field effect on cytoskeleton and on the structure of chromatin in human cells. We used cell line of small lung carcinoma (A549) and the effects of magnetic field on cytoskeleton and higher-order chromatin structure were analyzed 96 h of magnetic field exposure. Magnetic field induction was Bm = 2 mT and the net frequency f = 50 Hz. In such affected and control cells the F-actin was estimated using FITC-conjugated Phalloidin and mitochondria were studied using MitoTracker (Molecular Probes). Images of cytoskeleton and genetic loci were acquired using confocal microscopy and analysis was performed by FISH 2.0 software. Slight morphological changes of F-actin filaments and mitochondria were observed in affected cells and nuclear condensation was found. |
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