Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci.

Authors

STEPANOVIC Srdjan VUKOVIC Dragana HOLÁ Veronika DI BONAVENTURA Giovanni DJUKIC Slobodanka CIRKOVIC Ivana RŮŽIČKA Filip

Year of publication 2007
Type Article in Periodical
Magazine / Source APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
MU Faculty or unit

Faculty of Medicine

Citation
Field Microbiology, virology
Keywords biofilm; staphylococcus; microtiter plate; quantification
Description The details of all steps involved in the quantification of biofilm formation in microtiter plates are described. The presented protocol incorporates information on assessment of biofilm production by staphylococci, gained both by direct experience as well as by analysis of methods for assaying biofilm production. The obtained results should simplify quantification of biofilm formation in microtiter plates, and make it more reliable and comparable among different laboratories.
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