Application of Aqueous Phase System and Hydrophilic Magnetic Microspheres for Isolation of Bacterial PCR-ready DNA from Dairy Products

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Authors	RITTICH Bohuslav ŠPANOVÁ Alena ŠÁLEK Petr TRACHTOVÁ Štěpánka HORÁK Daniel
Year of publication	2008
Type	Article in Proceedings
Conference	7th International Conference on the Scientific and Clinical Applications of Magnetic Carriers
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	DNA; isolation; PCR; aqueous phase system; magnetic microparticles; dairy products
Description	The isolation of PCR-ready DNA from dairy products by magnetic hydrophilic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-co-GMA)) microspheres in the presence of an aqueous phase system (composed of PEG 600 or PEG 6000 and NaCl)was studied. The results presented show that magnetic microparticles are suitable for the isolation of genomic DNA which can by used for PCR. Proposed method was applied to analysis of real dairy products.
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