Chip-based CE for avidin determination in transgenic tobacco and its comparison with square-wave voltammetry and standard gel electrophoresis
| Authors | |
|---|---|
| Year of publication | 2008 |
| Type | Article in Periodical |
| Magazine / Source | Chromatographia |
| MU Faculty or unit | |
| Citation | |
| Field | Biochemistry |
| Keywords | chip-based capillary electrophoresis; avidin; transgenic tobacco plants; voltammetry; SDS-PAGE |
| Description | Avidin transgenic plants are a potential tool for providing resistance against various species of insect pests due to the sequestration of vitamin H (biotin) in the plant from the insect pests. In this project we compared three techniques for avidin determination in transgenic tobacco plants, a novel chip-based capillary electrophoretic method (Experion), classical polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (SDS-PAGE) and a square wave voltammetric method using a carbon paste electrode. We determined that the automated chip-based capillary electrophoretic method is rapid, sensitive and the results obtained are well repeatable. The avidin content measured in transgenic tobacco leaves using chip-based capillary electrophoresis varied from 15 to 854 ng per mg of fresh mass depending on the individual plant. |