Genotypic versus phenotypic methods in detection of Listeria monocytogenes prosthetic joint infection
| Authors | |
|---|---|
| Year of publication | 2009 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Medical Microbiology |
| MU Faculty or unit | |
| Citation | |
| Field | Epidemiology, infectious diseases and clinical immunology |
| Keywords | PCR; 16S rRNA; Listeria monocytogenes; prosthetic joint infection |
| Description | An uncommon case of a severe prosthetic joint infection (PJI) in a 71-year-old immunocompetent woman is presented. Listeria monocytogenes was identified in two consecutive samples using broad-range PCR and sequencing, whereas cultivation remained negative in the first sample and streptococci of a non-GAS, non-GBS type were detected in the second one. This report demonstrates that the phenotypic approach may lead to misidentification of L. monocytogenes in a routine clinical setting. Molecular methods of pathogen detection might be useful when a rare and/or unexpected microorganism is present or the sample is collected during antibiotic treatment. |