Interleukin-8 receptor 2 (CXCR2) gene variability in patients with chronic periodontitis alone or in combination with type 2 diabetes mellitus in the Czech population

Authors

BOŘILOVÁ LINHARTOVÁ Petra KAVŘÍKOVÁ Denisa LUČANOVÁ Světlana POSKEROVÁ Hana VOKURKA Jan FASSMANN Antonín IZAKOVIČOVÁ HOLLÁ Lydie

Year of publication 2016
Type Conference abstract
MU Faculty or unit

Faculty of Medicine

Citation
Description Background: Periodontitis is a chronic inflammatory disease triggered by specific subgingival bacteria. Evidence suggests that diabetes mellitus (DM) may modulate periodontal tissue destruction, e.g. by reducing the polymorphonuclear leukocyte functions, including chemotaxis, adherence and phagocytosis. CXCR2 is a specific receptor for the interleukin-8 (IL-8) that is chemoattractant for neutrophils with an important role in the regulation of inflammatory response. Material and Methods: We analyzed CXCR2 +785C/T (rs2230054), +1208C/T (rs1126579) and +1440A/G (rs1126580) polymorphisms in patients with chronic periodontitis (CP) or CP with type 2 DM (T2DM+CP) and healthy controls and determined their associations with risk of diseases and occurrence of periodontal pathogens. Totally, 575 subjects (170 controls, 325 patients with CP and 80 subjects with T2DM+CP) were genotyped using methods based on polymerase chain reaction (PCR). Bacterial colonization (A. actinomycetemcomitans, P. gingivalis, P. intermedia, T. forsythia, T. denticola, P. micros, F. nucleatum) was investigated by a DNA-microarray kit. Results: Although no differences in the allele/genotype frequencies between controls and patients with CP or T2DM+CP were found, TCA haplotype significantly increased the risk of T2DM+CP (P<0.05, OR=2.21, 95%CI:1.11-4.40 vs. controls and P<0.05, OR=1.52, 95%CI:1.08-2.16 vs. CP alone). A. actinomycetemcomitans occurred significantly more frequently in men patients with CP positive for T allele of CXCR2 +1208C/T variant (61.8% vs. 38.6%, P<0.05; OR=2.57, 95%CI=1.14-5.79) or positive for C allele of CXCR2 +785C/T polymorphism (61.8% vs. 40.9%, P<0.05; OR=2.33, 95%CI=1.04-5.25). Conclusion: Our findings demonstrated that CXCR2 gene variability may be one of the risk factors for T2DM+CP.
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