Electrochemical and Spectral Behavior of MicroRNA (miR-34a-5p)

Investor logo

Warning

This publication doesn't include Faculty of Medicine. It includes Faculty of Science. Official publication website can be found on muni.cz.
Authors

VEČEŘOVÁ Aneta HUDCOVÁ Kristýna PILAŘOVÁ Iveta MASAŘÍK Michal TRNKOVÁ Libuše

Year of publication 2015
Type Article in Proceedings
Conference XXXV. Moderní elektrochemické metody
MU Faculty or unit

Faculty of Science

Citation
Field Electrochemistry
Keywords miR-34a-5p; human cancer; miDNA; cyclic voltammetry; linear sweep voltammetry; circular dichroic spectroscopy; mercury electrode
Description MicroRNAs are a family of small noncoding RNAs (21-25 nucleotides), negatively regulating gene expresion at the post-transcriptional level. Their functions are critical to normal cellular processes such as differentiation and apoptosis. recent studies demonstrated that deregulated miRNA expression contributes to the malignant phenotype and for this reason miRNAs play a key role as both diagnostic markers and therapeutic targets for many different tumor types (colorectal cancer, breast cancer, lung cancer, prostate cancer, etc.). Great attention is devoted to the study of miR-34a-5p related to head and neck cancer and also prostate cancer. This miRNA is a direct transcriptional target of p53 and its overexpression after p53 activation mediates some of the key tumor suppressive effects of p53 via induction of growth arrest, apoptisis or senescence. Our contribution is focused on the comparison of the structure of miRNA, based on ribose, and its miDNA analogues, based on deoxyribose and containing U or T in this sequence, by using voltammetric methods (cyclic voltammetry - CV or linear sweep voltammetry - LSV). The voltammetric experiment was completed with CD (circular dichroism) spectra.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info