Analýza mikroRNA v krevní plazmě s cílem identifi kovat pacienty s duktálním adenokarcinomem pankreatu mající benefi t z chirurgické resekce


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Title in English Analysis of blood plasma micrornas to enable identification of patients with pancreatic ductal adenocarcinoma who will benefit from surgical resection


Year of publication 2019
Type Article in Periodical
Magazine / Source Klinická onkologie
MU Faculty or unit

Central European Institute of Technology

Keywords Biomarkers; Tumor; MicroRNAs
Description Background: Pancreatic ductal adenocarcinoma (PDAC) is the most common malignancy of pancreas, characterized by extremely poor prognosis largely due to problem with early dia gnosis and lack of progress in personalization of therapy. Of all available treatment strategies, radical surgical resection of the tumour in its early stage remains the only possibility how to reach long-term survival. However, even a technically perfect surgical resection may still not provide a survival benefi t for all PDAC patients. Appropriate selection of patients for surgical resection is one the important medical needs in management of PDAC patients. Material and methods: To this study we enrolled 24 PDAC patients who underwent surgical resection and preoperatively collected their blood plasma specimends. Patients were divided into to two prognostic groups according to their overall survival-12 patients with poor prognosis (median overall survival 10 months) and 12 patients with good prognosis (median overall survival 25 months). Small RNA sequencing technology was applied to screen for microRNAs (miRNA) with diff erential levels between both PDAC patient’s group. cDNA libraries were prepared using QIAseq miRNA Library Kit (Qiaqen) and sequencing by NextSeq500 instrument (Illumina). Results: When miRNA expression profi les of the PDAC patients from good and poor prognostic groups were compared, 61 miRNAs were identifi ed to have signifi cantly diff erent plasma levels between the two groups (p < 0.05). A total of 21 miRNAs showed increased expression and 40 miRNAs showed decreased expression in a group of patients with poor prognosis compared to patients with good prognosis. Conclusion: This study demonstrated diff erences in miRNA expression profiles in preoperative plasma specimens of PDAC patients with short and long overall survival. Our observations indicate that after independent validations plasma miRNAs might become useful bio markers for identifi cation of PDAC patients having clinical benefi t from surgical resection of the tumour.
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