Expression of chemotactic molecules in the choroid plexus following subarachnoid hemorrhage



Year of publication 2021
Type Appeared in Conference without Proceedings
Description Subarachnoid hemorrhage (SAH) is a subtype of hemorrhagic stroke. In our previous study we found dynamic immune cell response in the choroid plexus (CP) induced by SAH as well as increased intracranial pressure. The exact source of immune cells is not known. The aim of presented study was to assess the number of C-C chemokine receptor type 2 (CCR2) and C-X3-C motif chemokine receptor 1 (CX3CR1) positive cells, the expression of C-C motif chemokine ligand 2 (CCL2), C-X3-C motif chemokine ligand 1 (CX3CL1) and tumor necrosis factor ? (TNF?) in the CP in different time intervals after induction of SAH or application of artificial cerebrospinal fluid (ACSF). Our experiments were performed on 56 Wistar rats (males, 250g). SAH was induced by application of autologous blood or ACSF into the cisterna magna. The animals were then left to survive 1, 3 and 7 days after application. After time of survival, the SAH, ACSF and naive rats were perfused transcardially with Zamboni´s fixative. Coronal cryostat sections through the brains were cut and immunostained for CCR2, CCL2, CX3CR1, CX3CL1 and TNF?. Immunohistochemical staining showed that SAH leads to increased number of CCR2 positive cells 3 and 7 days following SAH as well as 3 days after application of ACSF when compared to naive animals. Increased number of CX3CR1 positive cells was found 3 days after induction of SAH. The amount of CCL2 as well as CX3CL1 did not shown any significant changes. Expression of TNF? was increased 3 and 7 days after induction of SAH or ACSF injection when compared with naive rats. In conclusion, our findings suggest that CCR2 and CX3CR1 positive cells invade the CP mainly 3 days following induction of SAH. TNF? may play an important role in chemotaxis of these cells.
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