Detekce profágů u TSST-1 pozitivních kmenů Staphylococcus aureus pomocí specifických sond

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Title in English Detection of prophages in TSST-1 positive strains of Staphylococcus aureus by means of specific probes


Year of publication 2000
Type Article in Proceedings
Conference Tomáškovy dny 2000 - sborník souhrnů přednášek
MU Faculty or unit

Faculty of Science

Field Genetics and molecular biology
Description The strains of Staphylococcus aureus producing TSST-1 toxin can induce human disease called Toxic Shock Syndrom (TSS). Production of staphylococcal toxins is based on the function of genes, which are usually localized in chromosome of host bacterium, but sometimes they can be a part of prophage genome. Temperate bacterophages of S. aureus can move genes by transduction and/or mediate lysogenic conversion of bacterial cells, which leads to increased ability of toxin production, usually of enterotoxins A and E. In this study 25 strains of S. aureus were analysed for presence of prophages of the serological groups A, B and F. The presence of prophages was determined using hybridization by means of specific probes to SmaI and HindIII DNA restriction patterns of S. aureus strains. Probes were prepared from genomic sequences of phages f77 (serological group F ), f53 (serological group B) and f3A (serological group A). Positive signal in SmaI restriction patterns was found in 92% strains when probe 77A was used. On the other hand probes 53B and 3A hybridized to 45% strains only. The presence of at least one of prophages (of three tested serological groups) was detected in HindIII restriction patterns of 96 % strains. The profages of serological group F were detected in 84% strains. Therefore we suppose that TSST-1 positive strains contain mostly prophages of serological group F. The occurence of prophages of serological groups A and B is less abundant. In conclusion we suppose that there is a certain correlation between presence of prophages of serological group F in the bacterial cells and their ability to produce TSST-1.
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