Odběr a zpracování vzorků pro expresní DNA čipy

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Title in English	Sample taking and processing for expression DNA microarrays
Authors	TICHÝ Boris SVOBODA M. MAYER Jiří POSPÍŠILOVÁ Šárka
Year of publication	2006
Type	Article in Periodical
Magazine / Source	Klinická onkologie
MU Faculty or unit	Faculty of Science
Citation
Field	Oncology and hematology
Keywords	RNA; microarray; biopsy; gene expression
Description	Gene expression profiling using DNA microarrays represents not only a powerfull tool for oncological research but have also a big potential to become a standard diagnostic technique. However, successfull microarray application needs to overcome some pitfalls such as sample collection, RNA isolation or nucleic acid labeling. Due to the number of microarray platforms there is no universal guide that can be used by all laboratories. The number of sometimes very different protocols for DNA microarray processing has a negative impact on their diagnostic utilization.