Substrate-Assited Laser Desorption Inductively-Coupled Plasma Mass Spectrometry for Elemental Detection in Microcolumn Effluent and Biological Submicroliter Samples

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Authors

PREISLER Jan JUNGOVÁ Pavla PEŠ Ondřej VACULOVIČ Tomáš NAVRÁTILOVÁ Jarmila ŠMARDA Jan KANICKÝ Viktor

Year of publication 2009
Type Conference abstract
MU Faculty or unit

Faculty of Science

Citation
Description A new method of sample preparation for elemental analysis using substrate-assisted laser desorption inductively-coupled plasma mass spectrometry (SALD ICP MS) is presented. Well-defined submicroliter volumes of analyzed samples are deposited onto polyethylene terephthalate glycol (PETG) plate and, after insertion into a commercial ablation cell, desorbed with pulses of 213-nm laser and analyzed with ICP MS. In contrast to conventional laser ablation (LA) ICP MS of solids, the analyzed compound is quantitatively desorbed during the PETG ablation. Furthermore, complete desorption is achieved at laser power density as low as ~10 MW/cm2, two orders below the levels typical in LA ICP MS. Model samples of metal salts in pg quantities were desorbed by less than 20 laser shots and relative spot-to-spot reproducibility was 3-10%. The limits of detection of Cr, Cu, Co, Fe, Ni, Sn and Zn deposited on the target were in the range of 50-500 fg. Selection of optimal conditions, such as laser fluence, number of laser shots, type of raster etc. will be discussed. The low limits of detection allow using SALD for off-line coupling of capillary electrophoresis (CE) to ICP MS.
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