Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

Authors

SLANINOVÁ Iva

Year of publication 2016
Type Article in Periodical
Magazine / Source Autophagy
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.1080/15548627.2015.1100356
Field Genetics and molecular biology
Keywords autolysosome; autophagosome; chaperone-mediated autophagy; flux; LC3; lysosome; macroautophagy; phagophore; stress; vacuole
Description In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes.

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