Průkaz bakteriálních agens z hemokultur metodou fluorescenční in situ hybridizace
| Title in English | Identification of bacteria from blood cultures by fluorescence in situ hybridization |
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| Authors | |
| Year of publication | 2017 |
| Type | Article in Periodical |
| Magazine / Source | Epidemiologie, mikrobiologie, imunologie |
| MU Faculty or unit | |
| Citation | |
| Field | Epidemiology, infectious diseases and clinical immunology |
| Keywords | sepsis; blood cultures; Gram stain; fluorescence in situ hybridization |
| Description | Altogether 71 samples of positive blood cultures were tested. Blood cultures were detected in BACTEC analysator, than inoculated on solid media and growing bacteria were biochemicaly identified. Conformity between both methods was not hundred per cent, sensitivity of HemoFISH was 90,1 %. Good results were observed in case of staphylococci and enterobacteria. FISH identification failed in three cases, because hybridization probe were not able to bind to bacterial rRNA. |