Subarachnoid hemorrhage induces reaction of M1 and M2 macrophages in choroid plexus



Year of publication 2018
Type Conference abstract
MU Faculty or unit

Faculty of Medicine

Description Introduction and purpose: The subarachnoid hemorrhage (SAH) is a specific form of the hemorrhagic stroke. Choroid plexus (CP) of the brain ventricles forms the blood-cerebrospinal fluid barrier (B-CSF-B) and is responsible for CSF production. The aim of our study was to describe the cellular changes characterized by the presence of pro-inflammatory (M1) macrophages and anti-inflammatory (M2) macrophages. Material and methods: Wistar rats (n=17; male; 250-300g) were used in experiment. SAH was induced by injection of autologous arterial blood (SAH group) or artificial CSF (control group) into the cisterna magna and rats were left to survive for 1, 3 and 7 days. After time of survival the SAH and control groups of animals were sacrificed in CO2, perfused transcardially by Zamboni´s fixative and the brain was removed. Coronal cryostat sections through the brain were cut and immunohistochemical detection of CCR7+ (M1) and CD206+ (M2) macrophages was performed. Numbers of M1+and M2+ cells per 1 mm2 of CP were counted and statistically analyzed. Results and conclusion: Number of M1+ macrophages was significantly increased 3 days after SAH comparing to naive and control rats. SAH induced significant increased number of M2+ macrophages after 3 and 7 days comparing to naive and control animals. Significant increased number of M2+ macrophages was found 7 days after injection of artificial CSF. The CP responds to SAH with initial increased number of M2+and M2+ macrophages 3 days after SAH. A later anti-inflammatory reaction characterized by presence of M2+ macrophages may contribute to restoration of B-CSF-B after SAH.
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