Applicability of capillary electrophoresis-frontal analysis for displacement studies: effect of several drugs on L-tryptophan and lidocaine binding to human serum albumin
| Authors | |
|---|---|
| Year of publication | 2020 |
| Type | Article in Periodical |
| Magazine / Source | Journa of Separation Science |
| MU Faculty or unit | |
| Citation | |
| Web | https://doi.org/10.1002/jssc.202000594 |
| Doi | http://dx.doi.org/10.1002/jssc.202000594 |
| Keywords | CE; Albumin; Drug |
| Description | In this study, the binding characteristics of six drugs with human serum albumin, the most abundant protein in human plasma, were determined by capillary electrophoresis-frontal analysis, and the obtained values of binding parameters were compared with the literature data. The affect of several drugs and site markers on the binding of L-tryptophan and lidocaine to human serum albumin was investigated in subsequent displacement studies which thus demonstrated the usability of capillary electrophoresis as an automated high-throughput screening method for drug-protein binding studies. |
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