Rozdiel účinku rifampicínu na staticky a dynamicky kultivovaný bakteriálny biofilm

Title in English The difference between the effect of rifampicin on a bacterial biofilm produced under static and dynamic cultivation conditions


Year of publication 2021
Type Conference abstract
MU Faculty or unit

Faculty of Medicine

Description The predominant mode of life of microorganisms is their coexistence in the form of biofilms. This is a relatively complex structure of cells, anchored in the extracellular polymer matrix (EPM), which makes up the majority of the structure and thanks to which the biofilm is attached to the surface. The presence of bacteria in the form of biofilms is beneficial, e.g. as part of the microbiome of mucous membranes, where it serves as protection against pathogens, or in industrial applications (wastewater treatment). In most cases, however, bacteria capable of forming biofilms are a major problem, especially in the treatment of bacterial infections. Biofilm infections are responsible for at least two-thirds of all infections and show strong resistance to classical antibiotic treatment Another problem is the colonisation of medical devices and equipment inserted directly into the human body, such as joint replacements, artificial heart valves, catheters, cannulas, etc. Many bacterial species have the ability to form biofilms. One of them is Staphylococcus aureus, which is the causative agent of purulent abscesses and causes serious complications in bloodstream infections leading to systemic diseases such as osteomyelitis or endocarditis. To approach the conditions of bacterial biofilm growth, this work focused on comparing the effect of rifampicin on biofilms cultured under different conditions. In the dynamic culturing, it was the flow chamber method. The advantages are the possibility of direct microscopic observation of biofilm formation and the easy control and management of the culture conditions. For static cultivation, the microtiter plate culture method was used. By quantifying the biofilm formed on the surface of the discs, the effect of rifampicin treatment on S. aureus biofilm cultured both statically and dynamically could be compared. It was found that in the case of rifampicin treatment with a concentration of 1.25 mg/ml, the loss of biofilm cultured in the flow chamber is approximately higher than in the case of static cultivation on the plate. At higher concentrations of rifampicin (5 and 2,5 mg/ml), there was no significant difference in biofilm loss between static and dynamic cultivation.
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