Antimikrobiálny účinok hydrogélu na báze Gum Karaye v kombinácii s fágovým preparátom na meticilín rezistentné kmene Staphylococcus aureus

Title in English Antimicrobial effect of Gum Karaya based hydrogel in combination with phage on methicillin-resistant strains of Staphylococcus aureus


Year of publication 2022
Type Appeared in Conference without Proceedings
MU Faculty or unit

Faculty of Medicine

Description Introduction Gum karaya (GK) is a natural polysaccharide with great potential in the treatment of chronic skin and soft tissue infections (SSTI). Polysaccharide-based hydrogels keep moist environment and accelerate wound healing. Their antimicrobial potential against Staphylococcus strains aureus resistant to methicillin has been previously established. Supplementing this material with a phage preparation, the effects can be enhanced. Aim To test the combination of the antimicrobial effect of GK and the phage preparation Methodology The phage preparation was first tested for its stability in GK solution and hydrogel. Then the release of phage particles from the GK hydrogel was studied. Antimicrobial potential of GK+BP was tested on a methicillin-resistant reference strain of S. aureus ATCC 43300 and on a set of 16 clinical strains. The defined bacterial suspension was added to GK+BP. Number of surviving cells was determined by serial dilution after 4, 8 and 12 hours of incubation. Results Stability testing of the phage preparation in GK solution and hydrogel demonstrated that GK substantially does not significantly affect phage activity. The study of the release of phage particles from the GK hydrogel showed more than 90 % release of phage particles from the hydrogel into solution. Antimicrobial testing GK+BP showed complete eradication of all bacterial strains tested within 12 hours in contrast to the GK hydrogel alone, which does not achieve complete eradication of bacteria. Conclusion These properties predispose GK hydrogels as a promising new material used in the treatment of SSTIs. GK+BP preparations have demonstrated effective eradication of infectious agents. The proliferation of phages at the site infection will further enhance the eradication effect.
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