Novel Reconstruction With Inter-Frame Motion Compensation For Fast Super-Resolution Live Cell Imaging



Year of publication 2022
Type Article in Proceedings
Conference 2022 IEEE International Conference on Image Processing (ICIP) 16-19 Oct. 2022
MU Faculty or unit

Faculty of Medicine

Keywords Image reconstruction; structured illumination microscopy (SIM); ADMM; optical flow
Description Structured illumination microscopy is a widely popular super-resolution technique for live cell imaging capable of surpassing the diffraction limit. Its temporal resolution is limited by the need to capture multiple low-resolution images to reconstruct a single high-resolution image. When observing rapid biological processes, the local movement between frames leads to the formation of reconstruction artifacts, which subsequently impair the data interpretation. We propose to include this type of movement in the definition of the image formation forward problem. The motion can then be estimated from the original data using optical flow, and the optimization problem is solved using the alternating direction method of multipliers. Our approach is tested against other reconstruction techniques on both synthetic and real biological data.
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