Recent stationary phase-based fractionation strategies in proteomic analysis
| Authors | |
|---|---|
| Year of publication | 2023 |
| Type | Article in Periodical |
| Magazine / Source | Journal of separation science |
| MU Faculty or unit | |
| Citation | |
| Web | https://doi.org/10.1002/jssc.202300174 |
| Doi | http://dx.doi.org/10.1002/jssc.202300174 |
| Keywords | liquid chromatography; offline; online; proteomics; multidimensional |
| Description | In this article, we review stationary phase-assisted sample prefractionation in proteomic analysis in 2019-2022 period. Applications are grouped according to the mode of retention used in the prefractionation step prior to the final analysis by low-pH reversed-phase liquid chromatography-tandem mass spectrometry. Online or offline instrumental configurations are also commented on, with a highlight on novel online platforms. From the articles reviewed in this period, the order of popularity of chromatographic modes for sample fractionation is affinity chromatography > size exclusion chromatography > hydrophilic interaction chromatography > high-pH reversed-phase liquid chromatography > ion exchange chromatography. |
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